0 and dispense 10 μL to all wells of the 384-well plate(s) (see Note 13). FlexStation® 3 Benchtop Multi-Mode Microplate Reader combines Molecular Devices’ SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. The fluorescent intensity of each well was measured by using a fluorescence plate reader (SpectraMax Paradigm, Molecular Devices, Sunnyvale, CA, USA) at 588-nm excitation and 633-nm emission. To maintain confidence in the standards, Molecular Devices recommends having validation plates recertified at one-to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, andSpectraMax® iD5 Multi-Mode Microplate Readers,6 to 384-Well Microplates, Molecular Devices, LLC (CAT#: STEM-LE-0504-LC) Product Keyword. x. 100 fmol luciferase per well. Molecular Devices, LLC. No accessories are needed and SoftMax® Pro Software. For a higher . A rinse program is included for quick and easy daily. luminescence for a 96-well plate and 40 msec integration time for a 384-well plate. the industry-leading microplate reader software for the SpectraMax Plus 384 system, allows complete customization of data collection and analysis. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. Use the provided 3. the Gemini XPS reader. Published Dec 16, 2020 | Updated Nov 07, 2022. 96-, 384-, and 1536. 2. 40266. DynaPro ™ Plate Reader III. Temperature Control: Ambient +5C up to 42C. 과 같은 Parameter를 검사하여 평가할 수 있습니다. Description. Contact Us Phone: +1-800-635-5577 Web: Email: info@moldev. A Costar® 24-well tissue culture plate with col1 = blanks and doubling cell concentration left to right starting at Col. A: adipocytes; B: fibroblasts; C: no cells (uptake reagent only). Applications include ELISA, protein quantification, enzyme kinetics and cell-based assays. ) 45 (W) x 70 (H) x 27 (D) Dimensions (cm) 114 (W) x 178. Prepare human iPSC-derived neuronal progenitor cells (NPCs) according to Shi et al. Microplate readers provide the ideal measurement platform for the high-throughput. 0. firefly luciferase in 96- and 384-well top read Dynamic range: > 6 decades Cross-talk: < 0. M5. 312 gain calibration failed. 0 . Table 3 shows sample cell proliferation assay results for CHO-K1 cells plated according to the scheme in Table 1 and allowed to proliferate in culture for two days. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. Molecular Devices’ SpectraMax® Plus 384 Microplate Reader provides fast and reliable full spectral range detection for cuvettes, 96-well and 384-well microplates. Once the assay runs its course, the final absorbance readout will take place on a SpectraMax® iD5 Multi-Mode Microplate Reader. It can be performed using any well in the plate with a relatively strong fluorescent signal (i. USA. Option 1: Use a plate. 2, prime the machine with CellTiter-Glo ® 2. This integrated system provides users with a multi-detection for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. 1: The SPECTRAmax 340PC 384 System The on-board microprocessor calculates and reports the absorbance (or % Transmittance) for each well of a microplate. Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. Contains one microplate adapter, five 24-well low volume slides, five 64-well low volume bottom slides, and five of each cover slide (2 µL and 4 µL) 0200-6267. ZERO BIAS - scores, article reviews, protocol conditions and more. FlexStation 3 Multi Mode Microplate Reader. We would like to show you a description here but the site won’t allow us. The same measurement settings can be used for subsequent plates as long as the volumes, tracer and concentrations remain the same. Application Area: Spectrophotometer for molecular biology. 384 well plate (Molecular Devices LLC) About; News;Disposable Pin Tool - 96 / 384 Head: Fixed Pin Tool - 384 Head: Grippable Pin Tool - 96 / 384 Head: Magnetic Pin Tool - 96 / 384 / 1536 Head: LUMINESCENCE MICROPLATE READING: Molecular Devices: SpectraMax L: Thermo Fisher Scientific: Luminoskan Ascent: MICROPLATE CYTOMETRY: Nexcelom Bioscience: Celigo S Cell Imaging. x. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. With the FLIPR 384 , you get the sensitivity, data fidelity, and real-time, kinetic information of the FLIPR I system in a 384-well mode. 0 out of 5 $ 14,499. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. 2. For the FlexStation 3 Reader - Keep the Black Plate Adapter in place fro both Top and Bottom reads. Visit the supplier page or submit a quote to learn more. Microplates, cuvettes (via adapter) Detectors. 384 wells, the SpectraMax iD3 reader. For FlexStation I/II/3 plate readers, what are the dead-volumes for the compound plate? The FlexStation plate readers assume a 1 mm pipette height from the bottom of the well when aspirating from a compound source plate. within each well. C. The patented quench technology is offered to drug discovery and life science researchers exclusively by Molecular Devices,. Filter 320/100 (available from Molecular Devices) Filter 616/10 (Molecular Devices cat. Users can also perform measurements such as endpoint, multiple wavelength, kinetic, linear and area scans. The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. Molecular Devices 1311 Orleans Drive Sunnyvale, California 94089 Telephone: +1-408. UV absorbance detection. Dispense 10 µL of each ATP/ADP combination across an entire row of a 384-well plate. SpectraMax iD3 reader with touchscreen and inserted USB flash drive. 1 OD at 230 nm. Values are averages from 5 clean plates, each filled with 200 µL water/well. Analyst, M5, Flex 3, and SpectraMax L readers work differently. x. Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. The SpectraMax 190 reader can be integrated with Molecular . 1. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax ® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. Industry standard disposable (choose from recommended list). Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. Microplates for applications that require high sample recovery rates. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384. *For Corning UV plates, mean values at 260 nm ranged from 0. 96- and 384-well . In these readers, RLUs are proportional to counts/sec which doesn't change with integration time assuming the. Simultaneous dual emission detection enables read times of only 2:17 for an entire 384-well plate. F3/F5 Multi-Mode Microplate ReadersBoth reactions can be easily measured in Molecular Devices’ new SpectraMax® L microplate luminometer. Outer wells aligned for proper viewing with FLIPR; Ideal for miniaturized 384 well fluorescent. 20 plates/hour (2-minute read time) 96-well 1,920 data points/hour 384-well 7,680 data points/hour 1536-well 30,720 data points/hour Bar code manual mode Keyboard entry Bar code remote mode Short or long side Temperature control Ambient +5°C to 40°C General specifications Dimensions (in. efficiency gratings, well scanning up to a 20x20 read matrix, spectral scanning and detection of plate formats from 6 to 384 wells, the SpectraMax iD3 reader is the complete solution. System setup and calibration are controlled from within SoftMax Pro Software. Microplate readers can be used to quantify this turbidity to monitor events such as cell growth or to perform solubility screens. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. 96-well (8 by 12 matrix) with a typical reaction volume between 100 and 200 µL per well. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. 8 mm distance between well bottoms to plate bottom; 50μL total well volume, with recommended working volume of 5-40μL; High base plate design is compatible with automation and for bar coding; Validated by Molecular Devices for the FLIPR reader. 1536-well plates < 100 amol (384-well) 1 HTRF • Cisbio HTRF detection with optimized Xenon light source and . 96- and 384-well. The 384-well plate is limited to a maximum volume of a 30 µl addition in a much smaller diameter well, and using the 96. Integrated stacker option. With optimized reagents, option to operate the readers. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. where I 0 is the intensity of the incident light, and I is intensity of that light after it passed through the sample. ©2017 Molecular Devices, LLC . They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. Experience the identifiably different. Ultra-cooled Photomultiplier Tube and : Silicon photodiode. x. . 1 fmol luciferase per well. formats from 6- to 384-wells, the SpectraMax iD3 reader is the complete solution for all your research needs. 25 µL/well in a 384-well plate, using AlphaScreen Phosphotyrosine (PT66) Assay Kit. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular biology and immunoassays. It is more inert and reduces compound interference. There is no product in the shopping cart, buy it!. (blue) and DsRed (red) transfected cells in 384-well plates, read from the bottom. Other technologies such as imaging. • Easy and gentle immobilization of biotin. Dynamic range > 6 decades . To prevent evaporation, the microplate was covered with a qPCR seal. #W50-100). EIGHT-CHANNEL OPTICS Plate reads as fast as five seconds MICROPLATES OR CUVETTES Use a 96- or 384-well microplate or a standard cuvette SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. Technical specifications. Guaranteed sensitivity: < 100 amol of biotinylated-LCK-P peptide, 25µL/well in a 384-well plate. Data for a 384-well plate can be collected in less than two minutes, as opposed to up to 30Molecular Devices Corporation (the “Seller”) shall not be liable for any incidental or consequential damages. Shaking: Linear, orbital, and double orbital. Inquiry Basket. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Streamline your workflow with comparative confluence assays to identify and verify gene edits. Total volume (except deep. Dynamic range > 6 decades . Catalog number: AB1384W. Abs). The plate reader consisted of a large-area. 96-, 384-, and 1536-well microplates. FlexStation 3 Benchtop Multi-Mode Microplate Reader User Guide Ordering Parts and Accessories To obtain parts and accessories, contact Molecular Devices Customer Service or your authorized service representative. Key benefits • Assess cell confluence objectively and quantitatively. 5 mm left a residual volume of 100 µL in each well of a 96-well plate or 25 µL for a 384-well plate. Plate formats. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. 4 Equipment. 1 or 10 Tablet PC with USB connection to instrument: Read Speed: 96-well plate: less than 1 minute; 384-well plate: less than 3 minutes: Automation Compatibility: Hardware and software accessible to third-party hardware/software control, including LIMS. Outer wells are aligned for proper viewing with FLIPR. 039. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. CO 2 & O 2 control. 2. 96- and 384-well plates; Wavelength(s): 635 nm (classification), 532 nm (reporter) Citations:. measurements in compatible Molecular Devices® microplate readers. 00. Like conventional microplate readers, multimode microplate readers detect reaction signals from microtiter plates in order to perform assays such as. , San Diego, CA, USA) such that the. This application is highlighted by the example of a fully automated kinetic solubility screen in 384-well plate format. In addition, SoftMax Pro SoftwareBelow is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. x. • Tyrosine Kinase Plate Please contact your Molecular Devices sales representative. T = I/I 0 and %T = 100 (T). SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. This can increase throughput and efficiency, thus saving time and space in the lab. Method 1. All other trademarks herein are the property of Molecular Devices, Inc. Related products. run on the FlexStation 3 reader in 384-well microplate with the FLIPR® Calcium 4 Assay Kit. Dimensions 128 x 86 x 15 mm. SpectraMax® Plus 384 provides ultrafast, full spectral range. They are also fully compatible with the FlexStation 3 Multi-Mode Microplate Reader. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. 0 (or higher) Software; Methods Data acquisition using the Workflow Editor. TNF standard curve. Molecular Devices SpectraMax Plus 384 Absorbance Reader Details. Filter. M5. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. 0 mP 96-well. 0 . 8mm distance between well bottoms to plate bottom 50 µL total well volume, with recommended working volume of 5-40 µL High base plate design is compatible with automation and for bar coding Validated by Molecular Devices for the FLIPR reader. (1536-well plate) 2 µL (quartz cuvette), 4 µL (disposable cuvette). A control well was set up to verify the accuracy of the standard curve. All stand-alone instrument functions can be controlled using the software. Download PDF. Absorbance cuvette port. You should ensure that there is enough volume in compound plate wells to operate the IonWorks® system taking into account the dead volume. VIS absorbance detection. SpectraMax® Plus 384 microplate reader can run both standard spectrophotometer and microplate reader applications on the same instrument. Instructions for adding new plates: CSI/CP2: Add plate. In addition, SoftMax Pro Software After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. Aspirating and dispensing of wash buffer can occur simultaneously in all wells via AquaMax® 4000 Microplate Washer with a 96- or 384-well head. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. Overview of 384 Well SBS. What is the minimum well volume needed to run a 384 well plate? Minimum volume depends on the solution (curved vs. VMax . 100 µL (96-well assay) or 25 µL (384-well assay) of CellTiter-Glo reagent was added to each well. Instruments with a 96- or 384-well format will accommodate the most commonly used plates. REF (SpectraMax Plus 384 only) A reading of buffer, water, or air taken in the cuvette that is used as I 0 to calculate Absorbance or % Transmittance. Rating: 3. Validating the absorbance optical performance of your microplate reader is as simple as reading a plate. Read one sample or up to 384 samples in a single plate by using any standard cuvette, or 96- or 384-well microplate. 96- and 384-well microplates. Gemini EM Reader scans. Access this by going to. 2. Microplate readers are commonly used in life science laboratories. The washer is engineered to be simple to maintain and use, including features that reduce clogging and noise. ability to use as little as 2˜µL samples with 24- or 64-well plates. 1 a. 384-Well Fully Skirted Plates, Barcoded, including Armadillo 384-Well PCR plates. Instrument pricing. With the QuickSync client installed on your computer, your SpectraMax iD3 reader can push data to any computer within the same network. All stand-alone instrument functions can be controlled using the software. x. 세포신호전달은 세포가 환경에 반응하고 다른 세포와 소통할 수 있게 합니다…. Typical 4 min/ 384 2 min/384 8 min/1536 Throughput plate plate plate Fully enabled protocols = reduced setup time. 340 PC384. By measuring optical pathlength, it. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. Microarray scanners for one-fluor to multi-fluor [email protected] and Download Molecular Devices SPECTRAmax PLUS 384 operator's manual online. #ID5) HTRF Detection System (Molecular Devices cat. A = 2 - log 10 (%T). Align the two slots on the bottle holder with the two pegs on the rail. VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. The FLIPR Fluorometric Imaging Plate Reader system addresses the industry's requirements for higher throughput, minimized sample volume, and cost reduction. An HTRF assay measures the fluorescence energy transfer between molecules in close proximity. Determine concentration using the Beer-Lambert LawMolecular Devices’ FilterMax™ F5 Multi-Mode Microplate Reader was tested for compatibility with Life. Both formulations utilize Molecular Devices proprietary quench technology to enhance signal windows and yield acceptable Z-scores to screen a variety of targets, including TRP, ligand-, cyclic nucleotide- and voltage-gated channels. Figure 6. Data for a 384-well plate can be collected in less than two minutes, as opposed to up to 30The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Service Contracts, Preventative Maintenance and. Microplates, cuvettes (via adapter) Detectors. Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader What is the minimum well volume needed to run a 384 well plate? Minimum volume. To ensure accurate cell numbers for the cell standard curves, cells were assayed immediately. This unlocks the transport slide. #3635) Transferpette micropipette (Drummond Scientific cat. (1536-well plate) 2 µL (quartz cuvette), 4 µL (disposable cuvette). Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. Turn on Plate Reader and wait for it to complete its start-up routine. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. 2. Pick the best speed, sensitivity, and price for your needs. based microplate readers that have 6well to 38 4well microplate reading capability as well as a builtin absorbance and fluorescence cuvette port. for the SpectraMax Multi-Mode Microp late Reader, as well as all other Molecular Devices' microplate readers. 2 with 1000 cells. Select Update Driver. For biochemical assays, the washer removes unbound material and unreacted reagents. , a leading provider of high-performance life science solutions and a company of Danaher Corporation (NYSE:DHR), has today. FLIPR Tetra systems are fluorescence- and luminescence-based microplate readers with an integrated 1536-, 384- or 96-pipettor. #6590-0144; includes Enhanced TRF Module, excitation and emission filters). Round well design with a clear flat bottom 2. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set ofThe plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. 1. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). #PLUS 384) UV-transparent 96-well microplates (Corning cat. Contact our support team with your questions regarding Molecular Devices products and applications. #4TI-0655) Microplates. Published Dec 16, 2020 | Updated Dec 16, 2020. 2 OD at 200 nm; this decreased to 0. -Flex3 link -we don't have this) complements the Tecans, in that it has an internal 8- or 16-tip pipetting head and performs multi-label measurements [fluorescence, fluorescence resonance energy transfer (FRET) and chemiluminescence] in real-time in 96 or 384 well format. (or plate reader). The choice of microplate is a crucial yet often overlooked technical decision in HTS and quantitative biology. Analyst, M5, Flex 3, and SpectraMax L readers work differently. Successful targets include calcium channels, chemokine receptors, and transiently transfected receptors. Molecular Devices Spectramax. 384-Deep Square Well Plates. Select Yes at the warning. SLS & DLS: 2 µL quartz cuvettes 45 µL quartz cuvettes 4 mL generic glass cuvette DLS only:Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. However, for greater flexibility, some instruments can accommodate 6- to 1536-well plate formats. For more sample throughput, the system canPage 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. Monthly Plate Verification Check (Absorbance Validation Plate) Note: Monthly Plate Verification Check is maintained by Quality Control. Made from polypropylene with conical well profiles to. Introduction. Supersedes 1. x. #R8190 (10) vials of component A*Instruments with a 96- or 384-well format will accommodate the most commonly used plates. It offers extremely low absorbance background, excellent well to well uniformity, and zero percent cross talk. Category 2 and 3 microplate readers use gradient heating as well. After incubation, the assay plate is transferred to the seal-peeler to remove the seal from the assay plate and transferred to washing. Each kit is sufficient for 3 Calcium 6 assay plates and 3 Calcium 6-QF assay plates. Gemini EM Reader scans. in every plate. Compare specifications and request a quote from leading suppliers. All standard 96- and 384-well microplates, strip wells, and filter-bottom micro-plates can be used in the SPECTRAmax PLUS 384. 8 mm distance between well bottoms to plate bottom; 50μL total well volume, with recommended working volume of 5-40μL; High base plate design is compatible with automation and for bar coding; Validated by Molecular Devices for the FLIPR reader. Aspirating and dispensing of wash buffer can occur simultaneously in all wells via AquaMax® 4000 Microplate Washer with a 96-. Molecular Devices has developed the FLIPR Membrane Potential Assay Kits to maximize. This user guide provides detailed instructions, troubleshooting tips and technical specifications for these instruments. Cells toxicity was corrected for the culture medium background absorbance. 6-well tissue-culture treated (TC) plate, sterile. Unmatched throughput FLIPR Membrane Potential Assay Kits allow the elimination of wash steps and much shorter read times on the instrument. Test up to 3. Scan every well . For more sample throughput, the system canThe SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. A 96- and 384-well automated washer. 96/384 - For the SpectraMax 340PC384, press to select to read a 96-well plate or a 384- well plate. Comprehensive solution. #0200-7011) Instrument setup The results above revealed excellent detection limits and dynamic range in all three Molecular Devices microplate luminometers. Add to Cart. 384-well TC plate, Poly-d-Lysine, Flat Clear. for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. Multichannel processes: Costar 96-well (3300) and Greiner 384-well (781182/781185) microplates are validated for multichannel processes. 2/24/2015 Molecular Devices FlexStation 3 Russ Desnoyer Our lab needed a way to measure Calcium flux downstream of. x. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time. A2000 Full Wavelength Microplate Reader, 6 ~ 384 Well Plate, SHANGHAI PEIOU ANALYSIS INSTRUMENT CO. Some have optional microplate readers, allowing emission scans in up to 384-well plates, for example. Mix well by trituration or plate shaker and incubate for 2 to 5 minutes at room temperature, protected from light. RUO. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. Results Microplate readers read biological, chemical or physical events that occur on a microtiter plate. Plate readers avoiding cross-talk are recommended for luminescence measurement in 384 well plates. Well scans from the Gemini EM Reader illustrating serial dilution of CHO cells using the Live Cell assay from Molecular Probes Live/Dead (Calcein AM/Ethidium Homodimer-1) assay kit. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. 96/384-well plate formats. SpectraDrop High-Throughput Screening (HTS) Kit. “The ABS Plus reader comes standard with our patented PathCheck Technology which enables consistent results across a 96- or 384-well plate despite. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. The Raman plate reader was designed to simultaneously observe the Raman spectrum of 192 analytes loaded in 192 wells on a standard 384-well plate. com Check our website for a current listing of worldwide distributors. Selects either 96-well or 384-well plate for a stand-alone read. The SpectraMax ABS readers can be integrated with Molecular Devices StakMax® Microplate Stacker within . XML file. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. Pfa1 and Pfa1 Gpx4-KO cells stably overexpressing murine FSP1 were seeded on separate 384-well plates (500 cells per well), and screened with a library of small-molecule inhibitor compounds, as. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read:. #6590-0121) SpectraMax i3x® Multi-Mode Microplate Reader (Molecular Devices cat. There is no product in the shopping cart, buy it!. After reading the ELISA plate on both readers, each standard curve was nearly identical. Two high-energy xenon flash lamps: Reading capabilities. Artel MVS Bundle DM Std/800TSNB Reader Details. Working in microplates allows for smaller assays that use less sample and reagent and increases throughput. VMax . Note: SoftMax Pro Software version 6 and 7 also include a searchable help file within the software. Click SoftMax Pro Software User Guide. That explains the lower values on the LmaxII384 plate reader. The small-footprint Multiwash+ Microplate Washer quickly washes with both 96 and 384-well plates, has 20 standard wash protocols, and a simple LCD screen interface to customize speed, volume, soak time, and aspiration settings. flat-bottom: 50-60 µl. SpectraMax® Plus 384 Microplate Reader (Molecular Devices cat. 314 low lamp level warning. The narrow bandwidths used by the model increase measurement accuracy and linearity over a wide. A Costar 24-well tissue culture plate with col1 = blanks and doubling cell5014038F 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 11 Applications 12 DualPhotomultiplierTubes 12Reader Documentation Version No. 384 Well Plates; Wavelength(s): 200 to 1000 nm (Abs), 250 - 850 nm (Fluor) Specifications/Features: Shaking, Temperature control, Cuvette port; Read More. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. 0. The system comes with a single plate loading adapter and a choice of magazines for 20, 40 or 50 SBS-standard 96- and 384-well. Includes PC and software. x. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. SpectraMax ID5 Plate Reader (Molecular Devices). EPOCH2 Plate Reader/Spectrophotometer. Both 96- and 384-well assay formats yielded similar linearity and dynamic range, indicating the suitability of the dual-luciferase assay and SpectraMax i3x system for both formats. Reliable performance with a proven track record. 0 mm Holex HEXAGON ballhead bolt driver to loosen the screw located inside the Transport Lock opening until you feel the spring release. The SpectraMax Paradigm reader with HTRF Detection Cartridge offers high-throughput screening capability with excellent read times and Z’ factors. Molecular Devices makes. SpectraMax i3x Multi-Mode Microplate Reader (Molecular Devices cat. This chapter reviews key criteria for microplate selection, including: well number, well volume and shape,. CAPABILITIES OF THE EQUIPMENT: The high sensitivity, low-noise photomultiplier tubes (PMTs), and variable-volume injector configuration make this reader ideal for fast kinetic assays requiring. 7°C (gradient heating design). The all new CloneSelect Imager FL features high contrast multichannel fluorescent and white light imaging that allows for accurate single cell detection and proof of monoclonality at day 0. 340 PC384. 0112-0127C 9 Chapter1:Introduction TheFlexStation® 3Multi-ModeMicroplateReadercombinestheperformanceofadual-monochromator,multi-modemicroplatereaderandanintegrated8. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical. 96-, 384-, and 1536-well microplates. What plates do you recommend for SpectraMax Plate Readers? Luminescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. Technical specifications Detection limit for ATP Glow 0200-7012 (LUM) Optimized Guaranteed* 96-well (200 µL) 1. 7. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Application Area: Multi mode plate reader. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax ® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. FLIPR Calcium 4 Assay Kit (Molecular Devices, San Jose, CA, USA). We also prefer that fluorescence signals in one well don’t bleed into the other, as this means less background and more precise data. x. The plate was also read at z height 1 mm with varying delay and integration times for the donor and acceptor reads. For more sample throughput, the system canThe SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Programmable 10% to 100%: Sample Cell Options: 96-, 384- or 1536-well microwell plates. When the user applies a set temperature of 37°C, the top is heated to 37. SpectraMax® ABS Plus microplate reader can run both cuvette-based and microplate reader applications on the same instrument. The SpectraMax L reader is easily integrated with the optional StakMax microplate stacker from Molecular Devices for automated multiplate assays. 5 ng per well. Time Resolved Fluorescence: Solid white plates for top reads and white plates with clear bottoms for. Contact Us Phone: +1-800-635-5577 Web: Email: [email protected] nM Z Factor at EC. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Outer wells aligned for proper viewing with FLIPR. Get Quote . Can read 96 well plates (optional 384 well). x. x. The ImageXpress® Pico Automated Cell Imaging System is more than a digital microscope, combining high-resolution imaging with powerful analysis. 6% for the on-board optics (monochromators). In addition, SoftMax Pro SoftwareAfter incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. With the FLIPR 384 , you get the sensitivity, data fidelity, and real-time, kinetic information of the FLIPR I system in a 384-well mode. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate.